NHMW Data Repository

Historic museum samples provide evidence for a recent replacement of Wolbachia types in European Drosophila melanogaster.

DOI: https://doi.org/10.57756/ddvbqk
Creator(s):
Publication date: 25 Nov 2023
Publisher: Naturhistorisches Museum Wien (NHMW)
Collections: Central Research Laboratories
Instrument: ONT MinIon
Resource type: Dataset
License: CC BY
CC BY 4.0 International
Tags: Drosophila Evolutionary history Genomics Host symbiont interactions Museomics Wolbachia
Countries: Denmark, Finland, Germany, Portugal, Sweden
Taxa: Diptera, Rickettsiales
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https://doi.org/10.57756/ddvbqk

Strunov A., Kirchner S., Schindelar J., Kruckenhauser L., Haring E., Kapun M. (2023). Historic museum samples provide evidence for a recent replacement of Wolbachia types in European Drosophila melanogaster.. NHMW Data Repository. https://doi.org/10.57756/ddvbqk

Abstract

This dataset contains the newly assembled Wolbachia draft genomes and assembly statistics of eight previously published historic Drosophila melanogaster museum samples sequenced with Illumina (Shpak et al. 2023) and of nine previously unpublished contemporary and newly assembled Wolbachia draft genomes that were newly sequenced with Oxford Nanopore technology. All historic samples were comprised of wMelCS Wolbachia variants collected 200-90 years ago in Sweden, Denmark and Germany. The contemporary samples consisted of wMel and wMelCS variants from Portugal and Finland and of wMel, wMelCS and wMelPop variants in long-standing Drosophila lab stocks.

Usage Notes

Sequencing data of historic samples were generated  on an Illumina NovaSeq Sequencer and assembled with the SPAdes assembler; Sequencing data of contemporary Samples were sequenced on a ONT MinION sequencer and assembled with Flye. A detailed description of the analysis pipeline can be found in https://github.com/capoony/WolbachiaEvolHist_2023

Funding

Agency Program Proj. Id Proj. Title
FWF Austrian Science Fund Standalone Grant P32275 Keep cool: The Influence of Symbionts on Thermal Preference

Publications

Strunov A, Kirchner S, Schindelar J, Kruckenhauser L, Haring E, Kapun M. 2023. Historic museum samples provide evidence for a recent replacement of Wolbachia types in European Drosophila melanogaster. Molecular Biology and Evolution:msad258. https://doi.org/10.1093/molbev/msad258

Downloads

File Description Size
AssembledGenomes.zip is a zipped folder with all assembled genomes in gzipped FASTA format 5.35 MB
BlobTools_plots.zip contains BlobPlots for all genomes 9.54 MB
BUSCO_reports.zip contains all reports from the BUSCO analyses including all predicted gene models 363.35 MB
README.txt A README file with descriptions of the data 0 MB
SampleIDs.xlsx is a table with all synonymously used sample IDs 0.01 MB